WORKING WITH RECORDED IMAGES
LOADING INDIVIDUAL FILES
The Flamingo saves recorded image data as raw files, a fast and efficient format. Each stack is saved as a single file. Individual raw files can be imported into Fiji via File > Import > Raw… or by drag-and-drop. Adjust the following settings during import:
- Image type: 16-bit Unsigned
- Width & Height: 2048 (unless cropped)
- Number of images: ≥ number of planes
- Little-endian byte order
CONVERTING A LARGE NUMBER OF FILES
Click here to download a Fiji macro for converting all image files in a given experiment folder. This tool converts raw files recorded with a Flamingo microscope to tif files. Options for max projection, small preview, cropping and combining two illumination sides. Files are saved to subfolders.