Quantitative analysis (intensity-based)
- measure and compare fluorescence intensities within a sample, between samples, over time
- analyze colocalization, FRET
Quantitative analysis (structure-based)
- measure size, area, volume, distance
- track objects over time
Pretty image
- give an impression of what your specimen looks like
- use your image for postcards, to put on a wall, submit to image competitions
| quantitative analysis (intensity-based) | quantitative analysis (structure-based) | pretty image | |
|---|---|---|---|
| subtract background | (✓) | (✓) | ✓ |
| set gamma ≠ 1 | ✗ | ✓ | ✓ |
| change (reduce) bit-depth | ✗ | ✓ | ✓ |
| denoise | (✓) | (✓) | ✓ |
| sharpen | ✗ | (✓) | ✓ |
| color overlay (composite) | ✓ | ✓ | (✓) |
| color overlay (RGB) | ✗ | (✓) | ✓ |
| crop | ✓ | ✓ | ✓ |
| scale / change size | ✗ | (✓) | ✓ |
| z-projection | ✗ | (✓) | ✓ |
| lossy compression | ✗ | (✓) | ✓ |
| lossless compression | ✓ | ✓ | (✓) |