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DOS AND DON’TS

Quantitative analysis (intensity-based)

Quantitative analysis (structure-based)

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quantitative analysis (intensity-based)quantitative analysis (structure-based)pretty image
subtract background(✓)(✓)
set gamma ≠ 1
change (reduce) bit-depth
denoise(✓)(✓)
sharpen(✓)
color overlay (composite)(✓)
color overlay (RGB)(✓)
crop
scale / change size(✓)
z-projection(✓)
lossy compression(✓)
lossless compression(✓)

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