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Mechanoreceptive neurons in the spinal cord

Liz Haynes (Eliceiri Lab, University of Wisconsin-Madison)

Zebrafish
V-SPIM
488 nm
Nikon 25x/1.1
pco.edge 4.2
10 ms/plane

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Crustacean development

Carsten Wolff (Marine Biological Laboratory)

Parhyale hawaiensis
T-SPIM
405, 488 & 561 nm
Nikon 10x/0.3
pco.panda 4.2
25 ms/plane

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48 hours of Parallel development

Alyssa Graves (Huisken lab, Morgridge Institute for Research)

Zebrafish
V-SPIM
488 nm
Nikon 10x/0.3
pco.panda 4.2
25 ms/plane

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Vasculature and calcium levels in the brain

Armin Bahl (Engert Lab, Harvard University; now University of Konstanz)

Zebrafish
T-SPIM
488 & 561 nm, double-sided
Nikon 16x/0.8
pco.panda 4.2
12 & 100 ms/plane

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Cell membranes in an Early worm embryo

Julian Kimura (Srivastava Lab, Harvard University)

Hofstenia miamia
T-SPIM
561 nm
Nikon 16x/0.8
pco.panda 4.2
25 ms/plane

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Cell divisions

Kurt Weiss (Huisken lab, Morgridge Institute for Research)

Zebrafish
V-SPIM
488 nm
Nikon 25x/1.1
pco.panda 4.2
25 ms/plane

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Neutrophil migration in vasculature

Briana Rocha-Gregg (Huttenlocher Lab, UW-Madison)

Zebrafish
T-SPIM
561 nm
Nikon 10x/0.3
pco.panda 4.2
25 ms/plane

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Notochord, Intestine & bone

Jamie Garcia, Esther Park, Jennifer Bagwell & Brianna Peskin (Bagnat Lab, Duke University)

Zebrafish
T-SPIM
488 & 561 nm
Nikon 16x/0.8
pco.panda 4.2
50 ms/plane

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Calcium levels in hydra

Ali Hanson (Yuste Lab, Columbia University)

Hydra vulgaris
T-SPIM
488 nm
Nikon 10x/0.3
pco.panda 4.2
100 ms/plane

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Peripheral nervous system

Julie Jurgens (Engle Lab, Boston Children’s Hospital)

Zebrafish
T-SPIM
488 nm
Nikon 16x/0.8
pco.panda 4.2
100 ms/plane

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Springtail

Guilherme Gainett (Sharma Lab, University of Wisconsin-Madison)

Collembola
T-SPIM
405 nm
Nikon 10x/0.3
pco.panda 4.2
25 ms/plane

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Root tip growth

Cara Winter (Benfey Lab, Duke University)

Arabidopsis thaliana
T-SPIM
561 nm
Nikon 16x/0.8
pco.panda 4.2
100 ms/plane

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Early development

Anjalie Schlaeppi (Huisken Lab, Morgridge Institute for Research)

Zebrafish
T-SPIM
488 nm
Nikon 10x/0.3
pco.panda 4.2
100 ms/plane

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hydra development

Alyssa Graves (Huisken lab, Morgridge Institute for Research)

Hydra vulgaris
T-SPIM
488 nm
Nikon 10x/0.3
pco.panda 4.2
100 ms/plane

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Vasculature and blood flow

Alyssa Graves & Anjalie Schlaeppi (Huisken Lab, Morgridge Institute for Research)

Zebrafish
T-SPIM
488 & 561 nm
Nikon 10x/0.3
pco.panda 4.2
100 ms/plane

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Spherical colony

Alyssa Graves (Huisken Lab, Morgridge Institute for Research)

Volvox
T-SPIM
488 nm
Nikon 10x/0.3
pco.panda 4.2
25 ms/plane

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Stem cell migration into the kidney

Ji Wook Kim, Sam Wattrus & Elliott Hagedorn (Zon Lab, Boston Children’s Hospital)

Zebrafish
T-SPIM
488 & 561 nm, Brightfield
Nikon 16x/0.8
pco.panda 4.2
25 ms/plane

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Squid embryogenesis

Caroline Albertin (Marine Biological Laboratory)

Zebrafish
T-SPIM
488 & 561 nm, Brightfield
Nikon 10x/0.3
pco.panda 4.2
25 ms/plane

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Injected cells

Anjalie Schlaeppie (Huisken Lab, Morgridge Institute for Research)

Zebrafish
T-SPIM
488 nm, Brightfield
Nikon 10x/0.3
PCO Panda 4.2
25 ms/plane

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Embryo & adult

Duygu Özpolat (Marine Biological Laboratory)

Platynereis dumerilii
T-SPIM
488 nm, Brightfield
Nikon 10x/0.3
pco.panda 4.2
25 ms/plane

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Cell nuclei & migrating neurons

Anjalie Schlaeppi (Huisken Lab, Morgridge Institute for Research)

Zebrafish
T-SPIM
488 & 561 nm
Nikon 10x/0.3
pco.panda 4.2
50 ms/plane

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adult worm

Duygu Özpolat (Marine Biological Laboratory)

Pristina leidyi
T-SPIM
488 & 561 nm
Nikon 10x/0.3
pco.panda 4.2
12 ms/plane

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Fish skin

Nithya Ramkumar (Poss Lab, Duke University)

Zebrafish
T-SPIM
488 & 561 nm
Nikon 10x/0.3
pco.panda 4.2
100 ms/plane

Images and Movies recorded with Flamingo Light Sheet Microscopes

Mechanoreceptive neurons in the spinal cord

Crustacean development

48 hours of Parallel development

Vasculature and calcium levels in the brain

Cell membranes in an Early worm embryo

Cell divisions

Neutrophil migration in vasculature

Notochord, Intestine & bone

Calcium levels in hydra

Peripheral nervous system

Springtail

Root tip growth

Early development

hydra development

Vasculature and blood flow

Spherical colony

Stem cell migration into the kidney

Squid embryogenesis

Injected cells

Embryo & adult

Cell nuclei & migrating neurons

adult worm

Fish skin

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